Technique IRAP (inter retrotransposon amplified polymorphism) to study the genetic variability in Sclerotinia sclerotiorum populations
| Autor: | Mateus Ferreira Santana, Marisa Vieira de Queiroz, Tania Maria Fernandes Salomão, Pedro H S Pereira, Everaldo Gonçalves de Barros, Míriam Goldfarb |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Genetics education.field_of_study biology Population Sclerotinia sclerotiorum food and beverages Retrotransposon Plant Science biology.organism_classification Long terminal repeat White (mutation) 03 medical and health sciences 030104 developmental biology Polymorphism (computer science) Genetic variability education Pathogen |
| Zdroj: | Australasian Plant Pathology. 45:357-360 |
| ISSN: | 1448-6032 0815-3191 |
| DOI: | 10.1007/s13313-016-0421-8 |
| Popis: | Sclerotinia sclerotiorum, the causal agent of the white mold in dry beans, is responsible among other damages, for pronounced losses in bean cultivation. Therefore, there is an urgent need for greater research into the genetic variability of S. sclerotiorum. This study was done to evaluate the technical efficiency of IRAP (Inter Retrotransposon Amplified Polymorphism) to explore the genetic variability in this fungus. Primers were designing for the conserved regions of the long terminal repeats (LTRs) of the retrotransposons Copia-LTR_SS and Gypsy-LTR_SS. The primers were tested in the pathogen populations of S. sclerotiorum from Minas Gerais, Brazil. The estimated values of the high genetic variability indicate that the IRAP technique is a viable alternative and useful in population studies on the genetic variability of S. sclerotiorum. |
| Databáze: | OpenAIRE |
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