Effect of Adding Ions - Metal Ions to Pectinase Activity Isolation from Bacillus Subtilis on Bleaching Paper

Autor:	Diah Mardiana, Mardiana Prasetyani Putri, Sasangka Prasetyawan, Anna Roosdiana
Rok vydání:	2013
Předmět:	food.ingredient Chromatography biology Pectin Chemistry Metal ions in aqueous solution Bacillus subtilis biology.organism_classification Enzyme assay Ion food Biocatalysis Reagent biology.protein Pectinase Nuclear chemistry
Zdroj:	Natural B. 2:73-80
ISSN:	2301-4202 2088-4613
DOI:	10.21776/ub.natural-b.2013.002.01.11
Popis:	Pectinase enzyme used as biocatalyst to overhaul pectat compounds or pectin. Pectinase avtivity was affected by metal ions. The purpose of this study is to determine the effect of adding metal ions on pectinase activity and its inhibition type.. Determination of enzyme activity was based on galacturonic acid produced, which was analyzed by spectrophotometric using DNS reagent. Concentration of metal ions used i.e. Zn 2+ , Cu 2+ , Fe 3+ were 2-10 mM. The result showed that V m and K M number without ions were 161.29 µmol/mL minutes and 0.55%(w/v), respectively. While the presence of ions Zn 2+ , Cu 2+ , Fe 3+ were producing V mapp 147.06; 156.25 and 153.85µg.ml -1 min -1 , K M app of 0.56%; 0.63% and 0.65%, and also K I of 333,33; 41,38; 32,9. The inhibition to metal ions are non-competitif inhibition.
Databáze:	OpenAIRE
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