Pasteurella multocida subsp. multocida and P. multocida subsp. septica Differentiation by PCR Fingerprinting and α-Glucosidase Activity
| Autor: | Helen T. Fernandez, Ellie J. C. Goldstein, Diane M. Citron, Sharon Hunt Gerardo, M. C. Claros |
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| Rok vydání: | 2001 |
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| Zdroj: | Journal of Clinical Microbiology. 39:2558-2564 |
| ISSN: | 1098-660X 0095-1137 |
| DOI: | 10.1128/jcm.39.7.2558-2564.2001 |
| Popis: | Pasteurella multocida is composed of three subspecies that are often differentiated by fermentation of sorbitol and dulcitol. We studied 35 dulcitol-negative P. multocida isolates from infected dog and cat bite wounds, 16 of which yielded weak and/or conflicting fermentation reactions in Andrades sorbitol, thus making it difficult to distinguish between the two dulcitol-negative subspecies of P. multocida , i.e., P. multocida subsp. multocida and P. multocida subsp. septica . All isolates and two control strains were further analyzed using a PCR fingerprinting technique with a single primer (M13 core) and assessed for α-glucosidase (α-Glu) activity. Although the PCR fingerprint patterns and α-Glu activity did not correlate well with the sorbitol fermentation reactions, they did correlate well with each other. All strains identified as P. multocida subsp. septica were positive for α-Glu activity and exhibited the group I PCR fingerprint profile. All strains categorized as P. multocida subsp. multocida displayed either the group II or group III PCR fingerprint profile; 9 of 11 of these isolates were α-Glu negative. These data suggest that both PCR fingerprinting and α-Glu activity provide reliable means for differentiating P. multocida subsp. multocida from P. multocida subsp. septica , particularly in strains that produce weak and/or discrepant sorbitol fermentation reactions. |
| Databáze: | OpenAIRE |
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