Intrafemoral Delivery of Hematopoietic Progenitors
| Autor: | Lai Guan Ng, Immanuel Kwok, Maximilien Evrard |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
0301 basic medicine
Adoptive cell transfer medicine.diagnostic_test Cell Cell sorting Biology Cell biology Flow cytometry 03 medical and health sciences Haematopoiesis 030104 developmental biology 0302 clinical medicine medicine.anatomical_structure 030220 oncology & carcinogenesis medicine Bone marrow Stem cell Progenitor cell |
| Zdroj: | Methods in Molecular Biology ISBN: 9781071614242 |
| Popis: | Hematopoiesis is a central process and is essential for the replenishment of short-lived leukocytes such as neutrophils. However, the molecular events underlining the developmental transition of quiescent hematopoietic stem cells into downstream progenitors and mature blood cells are not completely understood. Here, we describe the intrafemoral delivery of hematopoietic progenitors as a method to trace their development and differentiation lineage patterns within the bone marrow (BM) niche. Unlike other approaches, the direct adoptive transfer of progenitors into the BM cavity does not require prior irradiation preconditioning of recipient mice, and enables the delivery of lower cell numbers into the marrow space in a minimally perturbed environment. As a demonstrative example, we provide a protocol for the isolation of granulocyte-monocyte progenitors (GMP) by cell sorting, the delivery of these cells into recipient animals by intrafemoral transfer, and finally, the analysis of GMP-derived progenies by flow cytometry. |
| Databáze: | OpenAIRE |
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