A Gβ-like protein from soybean

Autor:	Sergei Reverdatto, Vadim Beilinson, Ronald Bassüner, Niels C. Nielsen
Rok vydání:	2001
Předmět:	Messenger RNA Physiology G protein Binding protein food and beverages RNA Cell Biology Plant Science General Medicine Biology Biochemistry Gene expression Protein A/G Genetics biology.protein Gene Peptide sequence
Zdroj:	Physiologia Plantarum. 111:75-82
ISSN:	1399-3054 0031-9317
DOI:	10.1034/j.1399-3054.2001.1110110.x
Popis:	A cDNA clone that encodes a G β -like protein was isolated from germinating soybeans. The deduced amino acid sequence for this protein is similar to other G β -like proteins isolated from plants and animals and contains 7 copies of the WD-40 repeat. RNA blots and reverse transcriptase polymerase chain reaction (PCR) revealed that mRNA for the soybean G β -like protein is present during seed development and germination, periods during seed ontogeny that are associated with high levels of biosynthetic activity. DNA blot analysis suggested there are only one or two copies of this gene in the soybean genome. Antibodies were raised against recombinant soybean G β -like protein expressed in bacteria. The antibodies reacted with a 35-kDa soybean protein that was present in minor amounts in developing seeds. The precise functions of G β -like proteins are unknown, but there are indications that they are involved in signal transduction pathways and in this way participate in control of seed growth and development.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::b4220a031269b4f43d93cccf1210ba92 https://doi.org/10.1034/j.1399-3054.2001.1110110.x Zobrazit plný text záznamu Plný text