| Autor: |
R.J. Fido, Eric J. Hewitt, B.A. Notton |
| Rok vydání: |
1983 |
| Předmět: |
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| Zdroj: |
Plant Science Letters. 29:107-113 |
| ISSN: |
0304-4211 |
| DOI: |
10.1016/0304-4211(83)90029-9 |
| Popis: |
Steptoe cultivar barley plants and 2 azide-induced nitrate reductase-less mutants Az 12 and Az 13 were grown by nutrient flow technique with complete nitrate-nitrogen nutrient. Molecular weight distribution of cytochrome c reductases from 6-day-old plants showed that the 8.1 S component form accounted for only 13% (Az 12) or 7% (Az 13) of their respective gross activities, compared to 72% (Steptoe cv.). Nitrate reductase was reconstituted when extracts of the mutants (as apoenzyme sources) were mixed with the molybdenum containing complex (MCC) obtained from semipurified spinach nitrate reductase. Activities increased from 1.9% up to 76% (Az) 12 and from 2.5% up to 62% (Az 13) of Steptoe cv. value, with diminishing values as plants aged. Nitrate reductase was also reconstituted using acid-treated extracts of the mutants (as MCC sources) mixed with apoenzyme from either molybdenum-deficient spinach plants grown with ammonium nitrate nutrient or present in extracts of Az 13 plants grown with both nitrate and molybdenum. Activities respectively up to 13% of Steptoe cv. or 7.3% of spinach grown with molybdenum were obtained. The mutants appear to have a weakness in their capacity to maintain nitrate reductase as stable aggregated protein, but to contain all the components of the enzyme. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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