An affinity chromatographic method for the preparation of bacterial lipoteichoic acids
| Autor: | Ronald J. Doyle, Ana Amoroso, Gabriel Osvaldo Gutkind, Breacya A. Washington |
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| Rok vydání: | 1996 |
| Předmět: |
Microbiology (medical)
Chromatography biology Chemistry Elution Size-exclusion chromatography Bacillus subtilis respiratory system biology.organism_classification Microbiology Streptococcus sobrinus carbohydrates (lipids) stomatognathic diseases chemistry.chemical_compound Distilled water Affinity chromatography Glycerol lipids (amino acids peptides and proteins) Lipoteichoic acid Molecular Biology |
| Zdroj: | Journal of Microbiological Methods. 25:19-22 |
| ISSN: | 0167-7012 |
| Popis: | Lipoteichoic acids (LTAs) have the ability to form stable complexes with polysaccharides in ethanol solutions. When crude preparations of LTA from Bacillus subtilis and Streptococcus sobrinus were poured over a column of Sephacryl S-200 equilibrated with 80% ethanol, the LTAs bound strongly to the column. Elution of the LTA was brought about by distilled water. The affinity-purified LTA was able to sensitize red cells to aggregation with anti-poly(glycerolphosphate). Chemical analyses showed that glycerol:phosphorus ratios were near unity. Contaminating protein with LTA seemed to be very low when the affinity method was used. Yields of LTA, however, are only 40–50% of the conventional phenol extract-Sepharose gel filtration method. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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