Interaction of ascaridole, carvacrol, and caryophyllene oxide from essential oil ofChenopodium ambrosioidesL. with mitochondria inLeishmaniaand other eukaryotes
Autor: | Thomas Rosenau, Markus Bacher, Lars Gille, Sritama De Sarkar, Gerald Geroldinger, Mitali Chatterjee, Katrin Staniek, Sophie Bergmann, Ramón Scull, Matthias Tonner, Lianet Monzote |
---|---|
Rok vydání: | 2018 |
Předmět: |
0301 basic medicine
Pharmacology biology Superoxide 030106 microbiology Chenopodium ambrosioides Mitochondrion Leishmania biology.organism_classification law.invention 03 medical and health sciences chemistry.chemical_compound 030104 developmental biology chemistry Biochemistry law Coenzyme Q – cytochrome c reductase Carvacrol Ascaridole Essential oil |
Zdroj: | Phytotherapy Research. 32:1729-1740 |
ISSN: | 0951-418X |
Popis: | The antileishmanial activity of the essential oil (EO) from Chenopodium ambrosioides L. has been demonstrated in vitro and in animal models, attributed to the major components of the EO. This study focused on the effects of the three major EO compounds carvacrol, caryophyllene oxide (Caryo), and the antileishmanial endoperoxide ascaridole (Asc) on mitochondrial functions in Leishmania tarentolae promastigotes (LtP). EO and Caryo were able to partially inhibit the leishmanial electron transport chain, whereas other components failed to demonstrate a direct immediate effect. Caryo demonstrated inhibition of complex III activity in LtP and in isolated complex III from other species. The formation of superoxide radicals was studied in Leishmania by electron spin resonance spectroscopy in the presence of iron chelators wherein selected compounds failed to trigger a significant immediate additional superoxide production in LtP. However, upon prolonged incubation of Leishmania with Asc and especially in the absence of iron chelators (allowing the activation of Asc), an increased superoxide radical production and significant impairment of mitochondrial coupling in Leishmania was observed. Prolonged incubation with all EO components resulted in thiol depletion. Taken together, the major components of EO mediate their leishmanicidal activity via different mitochondrial targets and time profiles. Further studies are required to elucidate possible synergistic effects of carvacrol and Asc and the influence of minor compounds. |
Databáze: | OpenAIRE |
Externí odkaz: |