| Autor: |
Bruna M. Valente, Antonio Edson R. Oliveira, Santuza M. R. Teixeira, Fernanda L.B. Muegge, Thais S. Tavares, Ashton T. Belew, Viviane Grazielle-Silva, Fabiano Sviatopolk-Mirsky Pais, Najib M. El-Sayed, Alessandra A. Guarneri, Wanessa M. Goes |
| Rok vydání: |
2020 |
| Předmět: |
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| DOI: |
10.1101/2020.07.07.189688 |
| Popis: |
SummaryTrypanosoma cruzi has three biochemically and morphologically distinct developmental stages that are programed to rapidly respond to environmental changes the parasite faces during its life cycle. Unlike other eukaryotes, Trypanosomatid genomes contain protein coding genes that are transcribed into polycistronic pre-mRNAs and control of gene expression relies on mechanisms acting at the post-transcriptional level. Transcriptome analyses comparing epimastigote, trypomastigote and intracellular amastigote stages revealed changes in gene expression that reflect the parasite adaptation to distinct environments. Several genes encoding RNA binding proteins (RBP), known to act as key post-transcriptional regulatory factors, were also differentially expressed. We characterized one T. cruzi RBP (TcZH3H12) that contains a zinc finger domain, and whose transcripts are upregulated in epimastigotes compared to trypomastigotes and amastigotes. TcZC3H12 knockout epimastigotes showed decreased growth rates and increased capacity to differentiate into metacyclic trypomastigotes. Comparative transcriptome analysis revealed a TcZC3H12-dependent expression of epimastigote specific genes encoding amino acid transporters and proteins associated with differentiation (PAD), among others. RNA immunoprecipitation assays showed that transcripts from the PAD family interact with TcZC3H12. Taken together, these findings suggest that TcZC3H12 positively regulates the expression of genes involved in epimastigote proliferation and also acts as a negative regulator of metacyclogenesis. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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