Poly(ethyleneimine)-Mediated Large-Scale Transient Gene Expression: Influence of Molecular Weight, Polydispersity and N -Propionyl Groups
| Autor: | Zuzana Kadlecova, Lucia Baldi, David L. Hacker, Florian M. Wurm, Sophie Nallet, Harm-Anton Klok |
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| Rok vydání: | 2012 |
| Předmět: |
chemistry.chemical_classification
Polymers and Plastics Chemistry Dispersity technology industry and agriculture Ethyleneimine Bioengineering macromolecular substances Transfection Polymer Gene delivery law.invention Biomaterials chemistry.chemical_compound law Polymer chemistry Materials Chemistry Biophysics Recombinant DNA Molar mass distribution DNA Biotechnology |
| Zdroj: | Macromolecular Bioscience. 12:628-636 |
| ISSN: | 1616-5187 |
| DOI: | 10.1002/mabi.201100404 |
| Popis: | Three synthesis lots of linear poly(ethyleneimine) (PEI) are compared to a fully hydrolyzed linear PEI (commercially available as PEI "Max") regarding structure, polyplex formation with plasmid DNA, and transfection of suspension-adapted HEK-293E cells. PEI "Max" binds DNA more efficiently than the other PEIs, but it is the least effective in terms of transient recombinant protein yield. One PEI lot is fractionated by means of SEC. The fractions of high-M(n) PEI are the most efficient for complex formation and transfection. Nevertheless, the highest transient recombinant protein yields are achieved with unfractionated PEI. The results demonstrate that the polydispersity and charge density of linear PEI are important parameters for gene delivery to suspension-adapted HEK-293E cells. |
| Databáze: | OpenAIRE |
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