Reverse phase HPLC fractionation of the oligosaccharide alditols isolated from an I-active ovarian cyst mucin glycoprotein

Autor:	C.Allen Bush, Yu-Teh Li, Virendra K. Dua, Volker E. Dube
Rok vydání:	1985
Předmět:	chemistry.chemical_classification Chromatography biology Elution Cell Biology Reversed-phase chromatography Fractionation Oligosaccharide Biochemistry High-performance liquid chromatography Fucose Endoglycosidase chemistry.chemical_compound chemistry Galactose biology.protein Molecular Biology
Zdroj:	Glycoconjugate Journal. 2:17-30
ISSN:	1573-4986 0282-0080
DOI:	10.1007/bf01225110
Popis:	We have used reverse phase high pressure liquid chromatography (HPLC) to separate the reduced oligosaccharides produced by alkaline borohydride degradation of ovarian cyst blood group substances. From a single cyst, six oligosaccharides, ranging from two to seven residues in length, have been isolated by preparative HPLC on C-18 stationary phases using water for elution. The purity of the products and their structures were determined by high field proton NMR spectroscopy in conjunction with exo-and endoglycosidase digestion. All the chains isolated terminated inN-acetylgalactosaminitol which was substituted at the 3-postion by galactose and in some cases at the 6-postion byN-acetylglucosamine. The largest identified oligosaccharide was a heptasaccharide alditol containing a single α-linked fucose in a Lewis blood group structure (Lea).
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::b40479eac3a66d1fa907e827b732b642 https://doi.org/10.1007/bf01225110 Zobrazit plný text záznamu Full text from SpringerLink