Chemistry of some fluorescamine-amine derivatives with relevance to the biosynthesis of benzylpenicillin by fermentation
| Autor: | Greg T. Lonergan, Warren L. Baker |
|---|---|
| Rok vydání: | 2002 |
| Předmět: |
chemistry.chemical_classification
Chromatography biology Renewable Energy Sustainability and the Environment Chemistry General Chemical Engineering Organic Chemistry Phenylacetic acid Fluorescamine Penicillium chrysogenum biology.organism_classification Pollution Benzylpenicillin Corn steep liquor Amino acid Inorganic Chemistry Penicillin chemistry.chemical_compound Fuel Technology medicine Fermentation Waste Management and Disposal Biotechnology medicine.drug |
| Zdroj: | Journal of Chemical Technology & Biotechnology. 77:1283-1288 |
| ISSN: | 1097-4660 0268-2575 |
| DOI: | 10.1002/jctb.706 |
| Popis: | The reaction of fluorescamine with 6-aminopenicillanic acid (6-APA) at pH 4 was investigated for its potential use in the biosynthesis of benzylpenicillin. A number of amino acids and penicillin amine derivatives, that reacted with fluorescamine at pH 7–9, were unlikely to do so to a significant extent at pH 4 and hence were unlikely to interfere in results that led to the biosynthesis of benzylpenicillin. Biosynthesis was followed using Penicillium chrysogenum Wis F3-64, growing in a corn steep liquor medium in a shake flask fed with phenylacetic acid daily. Analysis of benzylpenicillin formation was effected enzymically and fluorimetrically. A sample of the fermentation broth was treated with buffer at pH 7.8 and an active penicillin acylase solution for 1 h at 37 °C. The pH was then lowered to 4 by swamping with acetate buffer and the solution was treated with fluorescamine. The resulting fluorescence was compared with that of a standard 6-aminopenicillianic acid solution treated in the same manner. In this case the biosynthesis of benzylpenicillin was found to increase over 6 days. The results were compared with those for a control broth where the penicillin acylase was not added. © 2002 Society of Chemical Industry |
| Databáze: | OpenAIRE |
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