Pyramiding of bacterial blight resistance genes in rice: marker-assisted selection using RFLP and PCR
| Autor: | G. Zhang, Sonam Singh, John Bennett, J. Domingo, Ning Huang, Gerard Magpantay, E. R. Angeles, Gurdev S. Khush, N. Kumaravadivel |
|---|---|
| Rok vydání: | 1997 |
| Předmět: |
Genetics
Germplasm Oryza sativa business.industry fungi food and beverages General Medicine Biology Marker-assisted selection biology.organism_classification Biotechnology Xanthomonas oryzae Genetic marker Restriction fragment length polymorphism business Agronomy and Crop Science Gene Selection (genetic algorithm) |
| Zdroj: | Theoretical and Applied Genetics. 95:313-320 |
| ISSN: | 1432-2242 0040-5752 |
| Popis: | DNA marker-assisted selection was used to pyramid four bacterial blight resistance genes, Xa-4, xa-5, xa-13 and Xa-21. Breeding lines with two, three and four resistance genes were developed and tested for resistance to the bacterial blight pathogen (Xanthomonas oryzae pv. oryzae). The pyramid lines showed a wider spectrum and a higher level of resistance than lines with only a single gene. To speed up the gene pyramiding process and to facilitate future marker-aided selection, we developed PCR markers for the two recessive genes, xa-5 and xa-13, and used these to survey a range of rice germplasm. The results of the germplasm survey will be useful for the selection of parents in breeding programs aimed at transferring these bacterial blight resistance genes from one varietal background to another. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full text from SpringerLink