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Background: Trypanosoma evansi causes trypanosomosis or ‘surra’ in domestic and wild animals and is transmittedby haematophagus tabanid fly. For successful control of the disease, it is important to detect the carrier animalsfollowed by successive treatment. For this purpose a reliable serological test is useful for mass screening of theanimals. Methodology: In the present study, emphasis was given to address the need for the development of a massscreening serological test by using purified flagellar antigen of T. evansi for the detection of antibody against surra.Also a pilot sero-epidemiological study for trypanosomosis in different species using purified flagellar (FLA) antigenbased indirect enzyme linked immunosorbant assay (I-ELISA) was conducted and compared with standard CATT/T.evansi test. The diagnostic potentiality of purified FLA antigen based I-ELISA (FLA-I-ELISA) was evaluated using197 sera samples from field animals including cattle, buffaloes, horses and donkeys. Results: The test showed anoverall 14.72% sero positivity (S.P). Among cattle, S.P was found to be 18.03%, in buffalo 25%, in horse 6.25% andin donkeys 5.5%. Conclusion: Thus, epidemiological survey were done with purified FLA antigen based I-ELISAtest in Karnataka reveals that, buffalo samples showed the maximum sero positivity for trypanosomosis. |
