Optimization studies of components in enzymatic cholesterol reagents containing cholesterol oxidase fromNocardia erythropolis,Streptomyces sp, orPseudomonas fluorescens
| Autor: | Yaovaluk Teerajetkul, Porntip H. Lolekha |
|---|---|
| Rok vydání: | 1996 |
| Předmět: |
Microbiology (medical)
chemistry.chemical_classification Cholesterol oxidase Cholesterol Biochemistry (medical) Clinical Biochemistry Public Health Environmental and Occupational Health Nocardia Pseudomonas fluorescens Hematology Biology biology.organism_classification Streptomyces Medical Laboratory Technology chemistry.chemical_compound Enzyme chemistry Biochemistry biology.protein Immunology and Allergy Sodium Cholate Peroxidase |
| Zdroj: | Journal of Clinical Laboratory Analysis. 10:167-176 |
| ISSN: | 1098-2825 0887-8013 |
| DOI: | 10.1002/(sici)1098-2825(1996)10:4<167::aid-jcla1>3.0.co;2-7 |
| Popis: | Although enzymatic methods for serum cholesterol determination are widely used in clinical laboratories, little is known about the optimization of each component in enzymatic reagents. We investigated the optimal components in the reagents containing cholesterol oxidase isolated from Nocardia erythropolis, Streptomyces sp, or Pseudomonas fluorescens. The optimal components in the reagents are: cholesterol oxidase 250 (Nocardia erythropolis), 250 (Streptomyces sp), or 300 (Pseudomonas fluorescens) U/L, cholesterol esterase 200 U/L, peroxidase 10,000 U/L, sodium cholate 3 mmol/L, 4-aminoantipyrine 0.5 mmol/L, phenol 20 mmol/L, Triton X-100 2 mL/L, and phosphate buffer, pH 7.0. Lower reaction sensitivity and lower cholesterol linearity, 5 mmol/ L will suppress the reaction resulting in low cholesterol linearity. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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