Combination of dezincification carbon anhydrase with dansylamide for zinc biosensing

Autor:	Ansheng Zhang, Min Li, En-jie Sun, Li Hua
Rok vydání:	2015
Předmět:	inorganic chemicals chemistry.chemical_classification Chemistry Inorganic chemistry chemistry.chemical_element Infrared spectroscopy Zinc Fluorescence body regions Active center Enzyme Reagent biological sciences health occupations bacteria General Materials Science Biosensor Carbon
Zdroj:	Journal of Wuhan University of Technology-Mater. Sci. Ed.. 30:639-642
ISSN:	1993-0437 1000-2413
DOI:	10.1007/s11595-015-1203-9
Popis:	This work demonstrated quantitatively that DSA (dansylamide) and apo CA (dezincified carbon anhydrase) interacted with each other in the presence of Zn2+ (zinc ion). The fluorescence emission of DSA in the presence of Zn2+ and the intensity of fluorescence, which was proportional to the concentration of Zn2+, could be used in the measurement of Zn2+ concentration. Considering that Zn2+ was the active center of enzymes like CA (carbon anhydrase), two dezincification reagents were compared to ensure the validity of our method. A certain range concentration of Zn2+ could be measured by the reaction product of apo CA, DSA and Zn2+, where the measurement limitation was about 60 nmol/L. Interaction of CA with DSA was also studied with infrared spectrometry.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::b3bc43fa602c45d4b46947d1cf4ca8d0 https://doi.org/10.1007/s11595-015-1203-9 Zobrazit plný text záznamu Full text from SpringerLink