Catharanthus roseus L. extract downregulates the expression profile of motility-related genes in highly invasive human breast cancer cell line MDA-MB-231
| Autor: | Salizawati Muhamad Salhimi, Aiman Mohammed Yousif, Nagla Mustafa Eltayeb, Venugopal Balakrishnan, Mohammad Al-Amin |
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| Rok vydání: | 2020 |
| Předmět: |
0106 biological sciences
0301 basic medicine biology Motility Cell Biology Plant Science Catharanthus roseus biology.organism_classification 01 natural sciences Biochemistry Molecular biology Focal adhesion 03 medical and health sciences 030104 developmental biology Cell culture Invadopodia Genetics Animal Science and Zoology Viability assay KEGG Molecular Biology Gene Ecology Evolution Behavior and Systematics 010606 plant biology & botany |
| Zdroj: | Biologia. 76:1017-1032 |
| ISSN: | 1336-9563 0006-3088 |
| DOI: | 10.2478/s11756-020-00641-5 |
| Popis: | This study aimed to investigate the effect of Catharanthus roseus L. (C. roseus) leaf extract on the migration and invasion of MDA-MB-231 cell line and elucidate the molecular mechanisms of action. Effect of the extract on cell viability was evaluated by MTT (3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl2H-tetrazoliumbromide) assay. Anti-migratory and anti-invasive effects were evaluated using scratch and Transwell assays. Effect on the levels and activities of matrix metalloproteinase (MMP)-2 and MMP-9 was determined using ELISA and gelatin zymography. Furthermore, changes in the expression of 84 genes commonly involved in cell motility were assessed by Reverse Transcription-Polymerase Chain Reaction (RT-PCR) and cell motility RT2 profiler PCR array. Gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analyses were performed using DAVID. Using STRING and Cytoscape software, hub genes were determined. The extract significantly (p |
| Databáze: | OpenAIRE |
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