Biolistic transformation of Mucor circinelloides

Autor:	Gloria Angélica González-Hernández, J.F. Gutierrez-Corona, Luis Herrera-Estrella, V. Rocha-Ramirez, M.I.G. Roncero
Rok vydání:	1997
Předmět:	biology Auxotrophy Genetic transfer Plant Science biology.organism_classification Spore Microbiology chemistry.chemical_compound Transformation (genetics) Plasmid chemistry Mucor circinelloides Genetics Ecology Evolution Behavior and Systematics Bacteria DNA Biotechnology
Zdroj:	Mycological Research. 101:953-956
ISSN:	0953-7562
DOI:	10.1017/s0953756297003614
Popis:	Intact ungerminated spores or germinating swollen spores of Mucor circinelloides were biolistically transformed to leucine prototrophy with the autonomous replicating plasmids pLeu4 and pMCL1302. The former developmental stage was efficiently transformed with both plasmids. The existence of pLeu4 sequences, as selfreplicating elements within Leu + transformants was established by Southern analysis and by the recovery of plasmid pLeu4 in bacteria transformed with total DNA from M. circinelloides transformants. This is the first report of the transformation of intact cells of a member of the Zygomycetes.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::b3b30f6846a2b6051dd64e242c080b33 https://doi.org/10.1017/s0953756297003614 Zobrazit plný text záznamu Full Text from ScienceDirect