PHA-L lectin and carbohydrate relationship: conjugation with CdSe/CdS nanoparticles, radiolabeling and in vitro affinities on MCF-7 cells
| Autor: | Serhan Sakarya, Perihan Ünak, Cenk Selçuki, Altan Kara, E. Ilker Medine, Ozlet Akca |
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| Rok vydání: | 2013 |
| Předmět: |
chemistry.chemical_classification
biology Chemistry Health Toxicology and Mutagenesis Public Health Environmental and Occupational Health Lectin Nanoparticle chemical and pharmacologic phenomena Conjugated system Pollution In vitro Analytical Chemistry Sialic acid chemistry.chemical_compound Nuclear Energy and Engineering Biochemistry MCF-7 biology.protein Monosaccharide Radiology Nuclear Medicine and imaging Spectroscopy Conjugate |
| Zdroj: | Journal of Radioanalytical and Nuclear Chemistry. 299:807-813 |
| ISSN: | 1588-2780 0236-5731 |
| DOI: | 10.1007/s10967-013-2783-5 |
| Popis: | This research aims to investigate the interaction between phytohemagglutinin-L (PHA-L) and sialic acid, which is abundant on the breast cancer cell (MCF-7) surface and displays monosaccharide characteristics, by experimental and computational methods. Experimentally, CdSe/CdS nanoparticles (QDs) were synthesized; PHA-L was conjugated with QDs and labeled with 125I. Radiolabeling yield was found to be 97 ± 1.2 %. Afterwards, in vitro bioaffinities of radiolabeled PHA-L conjugated QDs have been investigated on MCF-7 cells and it has been observed that the cell incorporation increased with time. The results indicated that 125I labeled QD-PHA-L conjugates represent significant affinity on MCF-7 cells. In the second step of the study, the crystal structure of carbohydrate interaction surface of PHA-L was extracted from the crystal structure of PHA-L. The interactions between this surface and sialic acid were calculated by computational tools. These calculations revealed specific interactions between PHA-L and sialic acid. Semi-empirical methods, PM3 and AM1, were used in these calculations. Significant outcomes have been obtained from the experimental and computational studies and these results demonstrated that PHA-L may be an effective agent for imagining MCF-7 cells. |
| Databáze: | OpenAIRE |
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