Effects of light deficiency on the accumulation of saikosaponins and the ecophysiological characteristics of wild Bupleurum chinense DC. in China
| Autor: | Zhanwei Zhai, Yuan Jiang, Yihui Wang, Jirui Gong, Sha Xu, Min Liu, Yan Pan, Qinpu Luo, Lili Yang |
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| Rok vydání: | 2017 |
| Předmět: |
0106 biological sciences
0301 basic medicine Chlorophyll b Chlorophyll a Photoinhibition Photosynthetic efficiency Biology Photosynthesis biology.organism_classification 01 natural sciences 03 medical and health sciences chemistry.chemical_compound Light intensity 030104 developmental biology chemistry Compensation point Bupleurum chinense Botany Agronomy and Crop Science 010606 plant biology & botany |
| Zdroj: | Industrial Crops and Products. 99:179-188 |
| ISSN: | 0926-6690 |
| DOI: | 10.1016/j.indcrop.2017.01.040 |
| Popis: | Bupleurum chinense DC. is one of the most important traditional Chinese medicines. Among its primary pharmacologically active components, saikosaponins play a vital role. We investigated the ecophysiological characteristics, accumulation of secondary metabolites, and antioxidant system of wild plants at low light intensity in the forest understory at shady and sunny sites. Compared with plants at sunny sites, plants at the shady site were more abundant, had higher specific leaf area (SLA), a lower maximum electron transport rate ( J max ), higher triose phosphate utilization (TPU), and higher chlorophyll b content (Chlb), but lower net photosynthesis ( P n ), root and aboveground biomass, dark respiration rate ( R d ), light compensation point (LCP), chlorophyll a to b ratio, carotenoid content, and nonphotochemical quenching (NPQ). These features suggest that under low light conditions, the species developed larger leaves to catch more light energy through light-harvesting complex II to increase its photosynthetic efficiency (its ability to use weak light) and photosynthetic electron transport, thereby improving carbon metabolism and survival. The maximum quantum efficiency of photosystem II was about 0.8 at both sites; thus, photoinhibition was not a major problem. Contents of saikosaponins a and d and the total saikosaponin content (SSa, SSd, and SS total ) were much higher at the shady site, accompanied by increased superoxide dismutase (SOD), catalase(CAT), and peroxidases (POD) activity and decreased malondialdehyde (MDA). This suggested that low light activates antioxidant stress-response pathways, leading to increased SSa, SSd, and SS total . Our results suggest that B. chinense has strong shade tolerance and would be suitable for cultivation in an agroforestry system. |
| Databáze: | OpenAIRE |
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