The application of the flow cytometry for determination of the action of serotonin on the antigenic composition and amount of DNA of plague microbe cultured at 37°C
| Autor: | Youri A. Popov, Svetlana N. Klueva, Tatyana N. Schukovskaya, Tatyana A. Polunina, Vladimir N. Korsukov, Alexander L. Kravtsov |
|---|---|
| Rok vydání: | 2003 |
| Předmět: |
education.field_of_study
medicine.diagnostic_test biology Microorganism Population medicine.disease biology.organism_classification Bubonic plague Molecular biology Flow cytometry Microbiology chemistry.chemical_compound chemistry Yersinia pestis Polyclonal antibodies medicine biology.protein Serotonin education DNA |
| Zdroj: | SPIE Proceedings. |
| ISSN: | 0277-786X |
| DOI: | 10.1117/12.518632 |
| Popis: | The results have been obtained by flow cytometry showed the significant raise of fluorescence intensity of Yersinia pestis EV cells binding FITC-labelled plague polyclonal immunoglobins after 24 h cultivation in Hottinger broth pH 7.2 at 37 °C in the presence of increasing concentration serotonin (5-Hydroxytryptamine). The presence of serotonin (5-HT) in nutrient broth at concentration of 10 -5 M, 10 -8 M could modulate DNA content in 37 °C growing population of plague microbe. The effect of this action depended of 5-HT concentration. |
| Databáze: | OpenAIRE |
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