Preparation of matrix reference material of aflatoxin M1 in milk powder

Autor: Lei Yu, Xuqian Guo, Li Ying, Liu Hongbin, Yinghua Cai, Xue Han
Rok vydání: 2021
Předmět:
Zdroj: Accreditation and Quality Assurance. 26:77-90
ISSN: 1432-0517
0949-1775
DOI: 10.1007/s00769-021-01460-0
Popis: The preparation and characterization of a matrix reference material for aflatoxin M1 in milk powder is described. Milk powder samples containing aflatoxin M1 were found in the screened natural milk samples. The preparation of matrix milk powder samples was developed through steps by vacuum concentration, spray-drying, cooling, mixing, weighing and packaging, and irradiation. This paper has developed a method for the determination of aflatoxin M1 in milk powder by isotope dilution liquid chromatography–tandem mass spectrometry (ID-LC-MS/MS) with immunoaffinity extraction as the pretreatment method, which was validated and determined the content of aflatoxin M1 in milk powder accurately. The homogeneity was studied by analyzing in triplicate for their aflatoxin M1 contents by ID-LC-MS/MS using random sampling schemes. The short-term stability was evaluated at three different storage temperatures (4, 25 and 50 °C) for 0, 3, 5, 7, 14 days, the long-term stability study comprised of measurement after 0, 1, 3, 6, 9, 12 months after storage at 4 °C, and then, analyses were investigated by ID-LC-MS/MS. After the combination determination of 8 laboratories, the final quantity value and expanded uncertainty of matrix reference material of aflatoxin M1in milk powder were: 0.49 ± 0.11 μg/kg, k = 2, P = 0.95, and the matrix reference material is homogenous and stable at 4 °C for at least 12 months, which can provide quality assurance for the later detection accuracy.
Databáze: OpenAIRE
Externí odkaz: