Inter-batch quality differences in purified human prolactin

Autor:	J Armstrong, I G Worsley, V S Fang
Rok vydání:	1978
Předmět:	Antiserum Qualitative difference Chromatography Biochemistry (medical) Clinical Biochemistry Lactoperoxidase Radioimmunoassay Assay sensitivity Biology Prolactin
Zdroj:	Clinical Chemistry. 24:941-943
ISSN:	1530-8561 0009-9147
Popis:	During the past four years, we secured six batches of purified human prolactin for iodination by the same lactoperoxidase technique. Two of these batches gave us more satisfactory results in terms of assay sensitivity and consistency than did the others, regardless of the antiserum used. Gel filtration of 125I-labeled hormone revealed that these two batches were composed of at least 55% "small" prolactin while the other four batches contained from 20 to 68% "big" prolactin. This qualitative difference was the most important contribution to assay discrepancies.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::b34cb4fdcf13552706444c9157f5583a https://doi.org/10.1093/clinchem/24.6.941 Zobrazit plný text záznamu