Methods for the Administration of EDAR Pathway Modulators in Mice
Autor: | Holm Schneider, Michael Cheeseman, Sonia Schuepbach-Mallepell, Angela Dick, Denis J. Headon, Michele Vigolo, Christine Kowalczyk-Quintas, Mahya Eslami, Pascal Schneider |
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Rok vydání: | 2020 |
Předmět: |
0301 basic medicine
Ectodermal dysplasia Fetus integumentary system business.industry Stimulation Pharmacology medicine.disease 03 medical and health sciences Route of administration 030104 developmental biology 0302 clinical medicine medicine.anatomical_structure stomatognathic system Sweat gland medicine Ectodysplasin A Hypohidrotic ectodermal dysplasia Receptor business 030217 neurology & neurosurgery |
Zdroj: | Methods in Molecular Biology ISBN: 9781071611296 |
DOI: | 10.1007/978-1-0716-1130-2_12 |
Popis: | Genetic deficiency of ectodysplasin A (EDA) causes X-linked hypohidrotic ectodermal dysplasia, a congenital condition characterized by the absence or abnormal formation of sweat glands, teeth, and several skin appendages. Stimulation of the EDA receptor (EDAR) with agonists in the form of recombinant EDA or anti-EDAR antibodies can compensate for the absence of Eda in a mouse model of Eda deficiency, provided that agonists are administered in a timely manner during fetal development. Here we provide detailed protocols for the administration of EDAR agonists or antagonists, or other proteins, by the intravenous, intraperitoneal, and intra-amniotic routes as well as protocols to collect blood, to visualize sweat gland function, and to prepare skulls in mice. |
Databáze: | OpenAIRE |
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