Self-assembly, doxorubicin-loading and antibacterial activity of well-defined ABA-type amphiphilic poly(N-vinylpyrrolidone)-b-poly(d ,l -lactide)-b-poly(N-vinyl pyrrolidone) triblock copolymers
| Autor: | Ravi Kumar Gundampati, Kheyanath Mitra, Shikha Singh, Ankita Shukla, Biswajit Ray, Kalyan Ramesh, Medicherla V. Jagannadham, Dipankar Chattopadhyay, Nira Misra |
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| Rok vydání: | 2016 |
| Předmět: |
Chemistry
General Chemical Engineering N-Vinylpyrrolidone Chain transfer 02 engineering and technology General Chemistry 010402 general chemistry 021001 nanoscience & nanotechnology 01 natural sciences Ring-opening polymerization Micelle Fluorescence spectroscopy 0104 chemical sciences chemistry.chemical_compound Polymerization Polymer chemistry Amphiphile Copolymer 0210 nano-technology |
| Zdroj: | RSC Advances. 6:25864-25876 |
| ISSN: | 2046-2069 |
| Popis: | A series of ABA type well-defined amphiphilic poly(N-vinylpyrrolidone) (PNVP)-b-poly(D,L-lactide)-b-PNVP triblock copolymers have been synthesized via the combination of ring opening polymerization and xanthate-mediated reversible addition–fragmentation chain transfer polymerization, and analyzed by 1H NMR spectroscopy and gel permeation chromatography. Aggregation properties of these amphiphilic triblock copolymers have been revealed by fluorescence spectroscopy, transmission electron microscopy and dynamic light scattering, and supported by 1H NMR spectroscopy. Doxorubicin (DOX) has successfully been loaded into the block copolymer micelles with a loading efficiency of 37.5%. DOX-loaded PNVP51-b-PDLLA48-b-PNVP51 block copolymer showed sustained release within 36 h. Antibacterial properties of DOX-loaded micelles have been found to be significantly effective with respect to free DOX in terms of minimum inhibitory concentration, disk diffusion assay, growth curve, bacterial reduction and enzymatic assay based on in vitro studies. |
| Databáze: | OpenAIRE |
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