Separation and purification of six biosurfactant rhamnolipids by high-speed countercurrent chromatography utilizing novel solvent selection method
| Autor: | Shaojun Zhang, Wanqing Wu, Diyang Li, Qinggong Zheng |
|---|---|
| Rok vydání: | 2015 |
| Předmět: |
Solvent system
Chromatography 010405 organic chemistry Chemistry Process Chemistry and Technology General Chemical Engineering Rhamnolipid Filtration and Separation 02 engineering and technology General Chemistry 021001 nanoscience & nanotechnology Mass spectrometry 01 natural sciences 0104 chemical sciences Solvent chemistry.chemical_compound Countercurrent chromatography Selection method Solubility 0210 nano-technology Fermentation broth |
| Zdroj: | Separation Science and Technology. 51:673-680 |
| ISSN: | 1520-5754 0149-6395 |
| DOI: | 10.1080/01496395.2015.1117101 |
| Popis: | In this study, an original solvent selection method was developed for the separation of biosurfactant rhamnolipids from fermentation broth by high-speed countercurrent chromatography. For this method, calculations were based on solubility and functional group parameters that enabled the rapid selection of suitable solvent systems at no more than 10% of average time by traditional method. Based on selection results, a three-phase solvent system composed of n-hexane-methyl acetate-acetonitrile-water (2:2:2:5, v/v) was efficiently used for one-step separation of a mixture of six rhamnolipids. Thereby, RhaC10C10 (0.73 mg), RhaC10 (0.51 mg), Rha2C10 (0.12 mg), RhaC10C12 (1.26 mg), Rha2C10C10 (1.03 mg), and Rha2C10C12 (0.87 mg) were extracted from prepared Pseudomonas aeruginosa (100.0 mg) with purities of 96.37, 95.20, 91.25, 84.41, 89.8, and 90.26%, respectively. The purified compounds were identified by high-performance liquid chromatography/mass spectroscopy, plus 1H and 13C nuclear magnetic resonan... |
| Databáze: | OpenAIRE |
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