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Summary For microcirculation research there is a need for baseline data and feasibility protocols describing microcirculation of various organs. The aim of our study was to examine the reliability and reproducibility of sidestream dark-field (SDF) imaging within the renal cortical microcirculation in rats. Renal microcirculation was observed using SDF probe placed on the exposed renal surface via the upper midline laparotomy. Video sequences recorded intermittently in short apneic pauses were analyzed off-line by using AVA 3.0 software (MicroVision Medical, Amsterdam, the Netherlands). Results are expressed as mean (SD) or median (25–75% percentiles). We obtained 60 clear sequences from all recorded analyzable videos from all the animals. The total small vessel and all vessel density (in mm.mm–2) were (28.79 ± 0.40) and (28.95 ± 0.40), respectively. The perfused small and all vessel density were (28.79 ± 0.40) and (28.95 ± 0.40), respectively. The DeBacker Score was (19.14 ± 0.43), the proportion of perfused vessels was 100% (100–100%) and the microvascular flow index was 3.49 (3–3.75). We conclude SDF imaging provides a reliable method to examine the renal microvascular bed in vivo and thus can be used for the study of the renal cortical vascular network in various experimental diseases models and clinical settings. Lay Description This article aims to discuss the practicability of obtaining the baseline data and feasibility protocols needed to describe the microcirculation in various organs. In order to examine the reproducibility as well as the reliability of obtaining valid microcirculatory data of particular organs, the authors choose a renal cortical microcirculation in rats observed by using the sidestream dark field imaging (SDF). SDF method can be used non-invasively as well, especially in the sublingual area even in clinical research. However, in this study, the SDF probe is placed on the exposed renal surface. The subsequent analysis of all recorded videos is off-line using special software capable of image stabilization and semi-automatic evaluation of microcirculation. The program provides a number of output data, such as vascular density, vascular perfusion and blood velocity. The findings indicate that SDF imaging provides a reliable method of examining the renal microvascular bed in vivo and thus can be used to study the renal cortical vascular network in various experimental models of diseases and clinical settings. |
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