MICROBIAL METHANOGENESIS ASSOCIATED WITH BROWN AND BITUMINOUS COALS IN THE BOHEMIAN MASSIF: ISOTOPIC AND METAGENOMIC EVIDENCE

Autor: J. Šerá, P. Jirman, P. Parizek, J. Francu, Z. Vilhelm, M. Koutny, F. Koutny
Rok vydání: 2021
Předmět:
Zdroj: 30th International Meeting on Organic Geochemistry (IMOG 2021).
DOI: 10.3997/2214-4609.202134148
Popis: Summary Microbial methane occurrence associated with brown and bituminous coals in the Bohemian Massif is documented by chemical and isotopic composition and by metagenomics analysis. New methane shows were found recently in the Lower Miocene Most basin in the NW Czech Republic in drillings to deeper horizons with unmined coal seams. Gas evolved from formation water contains up to 27 % methane of δ13C-CH4 ‐73.1 ‰ and 33% of CO2 of δ13C-CO2 ‐8.0 ‰. The microbiological investigations found the presence of archaeal methanogens. In fluids and muds closer to surface microbial communities were found capable of degrading complex organic compounds and methane oxidation. Bituminous coals mined in the Upper Silesian Coal Basin in the NE Czech Republic contain gas with up to 94% of microbial methane of δ13C-CH4 ‐64 to -68‰ and up to 5% CO2 of δ13C-CO2 ‐14 to -47‰. The gas data suggest active and fossil methanogenesis, microbial methane oxidation associated with surface water infiltration and widespread sorption of both gases on coal seams. The bacterial communities in the bituminous coals are dominated by organisms specialized on fermentative degradation of complex organics considered as the prerequisite for microbial methanogenesis. Archaeal methanogens were identified by metagenomic analysis.
Databáze: OpenAIRE