Culture of Penicillium ulaiense in a batch-reactor: stoichiometric studies

Autor: Verónica Beatriz Rajal, Carlos Mario Cuevas
Rok vydání: 2007
Předmět:
Zdroj: World Journal of Microbiology and Biotechnology. 24:1081-1090
ISSN: 1573-0972
0959-3993
Popis: Penicillium ulaiense is a pathogenic, slow growing, fungus that affects citrus fruits post-harvest. This is the first study on the growth of this fungus with different carbon sources. The linear relations between the net conversion rates were used to make the first approach to simulate the system. To accomplish this, P. ulaiense was cultured in a bioreactor using three carbon sources: glucose, sucrose, and lactose. The mass balance for C was closed. A black box model was adopted to describe the system, represented by two macrochemical reactions: (1) formation of biomass and (2) combustion of the C-source. The experimental yield coefficient and the elemental and charge balances were used to determine the linear relations between the net conversion rates of the relevant substances involved and the stoichiometric coefficients. The reaction rates were determined as a function of the independent conversion rates. The energy balance was based on the description of three redox half-reactions: production of biomass, utilization of substrate, and electron transfer. The application of the second law of thermodynamics allowed the calculation of the maximal theoretical yield coefficient. The dissipated energy, the theoretical yield coefficient, the maintenance energies and the thermodynamic efficiencies were also calculated.
Databáze: OpenAIRE
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