Fluorescent/SERS dual-sensing and imaging of intracellular Zn2+

Autor: Wei Deng, Xingang Ren, Ma Yadan, Dan Li, Duan Huazhen, Fei Jiang
Rok vydání: 2018
Předmět:
Zdroj: Analytica Chimica Acta. 1038:148-156
ISSN: 0003-2670
DOI: 10.1016/j.aca.2018.07.020
Popis: A fluorescent and surface-enhanced Raman spectroscopy (SERS) dual-mode probe is developed for imaging of intracellular Zn2+ based on N-(2-(bis(pyridine-2-ylmethyl)amino)ethyl)-2-mercaptoacetamide (MDPA) modified gold nanoparticles (MDPA-GNPs). Benefiting from the chelation-enhanced fluorescence (CHEF) between MDPA-GNPs and Zn2+, the fluorescent intensities of MDPA-GNPs are substantially enhanced with the increment of Zn2+ concentrations, which can be clearly observed by the naked eye. Under physiological conditions, the probe exhibits a stable response for Zn2+ from 1 μM to 120 μM, with a detection limit of 0.32 μM in aqueous solutions. The resultant MDPA-GNPs can be used for ultrasensitive SERS detection of Zn2+ because of the strong inter-particle plasmonic coupling generated in the process of Zn2+-triggered MDPA-GNPs self-aggregation, with a low detection limit of 0.28 pM, which is eight order of magnitude lower than the United States Environmental Protection Agency (US EPA)-defined limit (76 μM) in drinkable water. More importantly, the proposed probe can be applied for efficient detection of intracellular Zn2+ with excellent biocompatibility and cellular imaging capability. Therefore, a highly sensitive and selective nanosensor has been demonstrated for both reliable quantitative detection of Zn2+ in aqueous solution and real-time imaging of intracellular Zn2+, suggesting its significant potential utility in bioanalysis and biomedical detection in the future.
Databáze: OpenAIRE
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