Interaction between theCaenorhabditis eleganscentriolar protein SAS-5 and microtubules facilitates organelle assembly
| Autor: | Nicola J. Dynes, Sarah Bianchi, Michel O. Steinmetz, Pierre Gönczy, Manuel Hilbert, Ioannis Vakonakis, Kacper B. Rogala, Sebastian A. Leidel |
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| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Organelle assembly biology Centriole Cell Biology Flagellum biology.organism_classification Cell biology 03 medical and health sciences 030104 developmental biology 0302 clinical medicine Microtubule Organelle Organelle biogenesis Molecular Biology 030217 neurology & neurosurgery Caenorhabditis elegans Centriole assembly |
| Zdroj: | Molecular Biology of the Cell. 29:722-735 |
| ISSN: | 1939-4586 1059-1524 |
| DOI: | 10.1091/mbc.e17-06-0412 |
| Popis: | Centrioles are microtubule-based organelles that organize the microtubule network and seed the formation of cilia and flagella. New centrioles assemble through a stepwise process dependent notably on the centriolar protein SAS-5 in Caenorhabditis elegans. SAS-5 and its functional homologues in other species form oligomers that bind the centriolar proteins SAS-6 and SAS-4, thereby forming an evolutionarily conserved structural core at the onset of organelle assembly. Here, we report a novel interaction of SAS-5 with microtubules. Microtubule binding requires SAS-5 oligomerization and a disordered protein segment that overlaps with the SAS-4 binding site. Combined in vitro and in vivo analysis of select mutants reveals that the SAS-5–microtubule interaction facilitates centriole assembly in C. elegans embryos. Our findings lead us to propose that the interdependence of SAS-5 oligomerization and microtubule binding reflects an avidity mechanism, which also strengthens SAS-5 associations with other centriole components and, thus, promotes organelle assembly. |
| Databáze: | OpenAIRE |
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