Enzyme immunoassay using a crossed-beam thermal lens technique
| Autor: | Deng Yanzhuo, Zhou Ping, Zeng Yun'e, E. Chen, Shen Jingkai |
|---|---|
| Rok vydání: | 2000 |
| Předmět: |
Detection limit
chemistry.chemical_classification Chromatography medicine.diagnostic_test biology Substrate (chemistry) Horseradish peroxidase Analytical Chemistry law.invention Lens (optics) Enzyme chemistry law Immunoassay medicine biology.protein Quantitative analysis (chemistry) Spectroscopy Beam (structure) |
| Zdroj: | Microchemical Journal. 64:257-262 |
| ISSN: | 0026-265X |
| DOI: | 10.1016/s0026-265x(00)00014-x |
| Popis: | An enzyme immunoassay based on the use of crossed-beam thermal lens detection is described. In this assay, poly- N -isopropylacrylamide, a water-soluble, thermally precipitating synthetic polymer, was used as a carrier to minimize non-specific binding. The enzyme substrate of the horseradish peroxidase that was employed was 3,3′,5,5′-tetramethylbenzidine. The color development of the enzyme–substrate reaction was stopped by SDS and Na 2 SO 3 to achieve a stable blue solution. The background reduction and stabilization made it possible to use a crossed-beam thermal lens technique as the measurement method. This method was demonstrated to be applicable by determination of hepatitis B surface antigen in human serum. A detection limit of 0.15 ng/ml was obtained. This was more sensitive than that of the commercially available ELISA method. |
| Databáze: | OpenAIRE |
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