Selective solvent extraction of ruberythric acid from madder roots and subsequent hydrolysis with β-glucosidase

Autor:	Setsuji Tone, Teruyuki Masawaki, Masahito Taya
Rok vydání:	1996
Předmět:	Chromatography Aqueous solution biology Extraction (chemistry) Aqueous two-phase system Alizarin biology.organism_classification Applied Microbiology and Biotechnology Anthraquinone chemistry.chemical_compound Hydrolysis chemistry Rubia cordifolia Enzymatic hydrolysis Biotechnology
Zdroj:	Journal of Fermentation and Bioengineering. 81:567-569
ISSN:	0922-338X
DOI:	10.1016/0922-338x(96)81483-8
Popis:	Selective solvent extraction of anthraquinones (alizarin and lucidin) and their primeverosides (ruberythric acid and lucidin-3-o-primeveroside) from madder roots could be achieved using two-phase extraction with chloroform (CHCl3) and potassium hydroxide aqueous solution (KOHaq). Ruberythric acid extracted to the aqueous phase could be selectively and completely converted to alizarin within 360 min by enzymatic hydrolysis with β-glucosidase at 50°C and at pH 5, reducing the inhibitory effect on the reaction caused by alizarin, which is not extracted in appreciable amounts into the aqueous phase, without appreciable hydrolysis of lucidin-3-o-primeveroside. It was also found that ruberythric acid extracted from madder hairy roots by CHCl3-KOHaq two-phase extraction was efficiently converted to alizarin in the same manner.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::b2e54fe05df0d106133747a39148e7e2 https://doi.org/10.1016/0922-338x(96)81483-8 Zobrazit plný text záznamu Full Text from ScienceDirect