Vβ1 + Jβ1.1 + /Vα2 + Jα49 + CD4 + T Cells Mediate Resistance against Infection with Blastomyces dermatitidis

Autor:	George S. Deepe, Marcel Wüthrich, Hanna I. Filutowicz, Bruce S. Klein, Holly L. Allen
Rok vydání:	2007
Předmět:	biology Blastomyces dermatitidis Immunology T-cell receptor T lymphocyte biology.organism_classification Microbiology Virology Cytosol Infectious Diseases Antigen Immunization Immunity Parasitology Receptor
Zdroj:	Infection and Immunity. 75:193-200
ISSN:	1098-5522 0019-9567
DOI:	10.1128/iai.01148-06
Popis:	Immunization with a cell wall/membrane (CW/M) and yeast cytosol extract (YCE) crude antigen from Blastomyces dermatitidis confers T-cell-mediated resistance against lethal experimental infection in mice. We isolated and characterized T cells that recognize components of these protective antigens and mediate protection. CD4 + T-cell clones elicited with CW/M antigen adoptively transferred protective immunity when they expressed a Vα2 + Jα49 + /Vβ1 + Jβ1.1 + heterodimeric T-cell receptor (TCR) and produced high levels of gamma interferon (IFN-γ). In contrast, Vβ8.1/8.2 + CD4 + T-cell clones that were reactive against CW/M and YCE antigens and produced little or no IFN-γ either failed to mediate protection or exacerbated the infection depending on the level of interleukin-5 expression. Thus, the outgrowth of protective T-cell clones against immunodominant antigens of B. dermatitidis is biased by a combination of the TCR repertoire and Th1 cytokine production.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::b2d97b75fd0624ae14eea498c78567eb https://doi.org/10.1128/iai.01148-06 Zobrazit plný text záznamu