Long-Term Antibacterial Performance and Bioactivity of Plasma-Engineered Ag-NPs/TiO2 Nanotubes for Bio-Implants
| Autor: | Won Seok Jeong, Doo Hoon Song, Soo-Hyuk Uhm, Kwang Mahn Kim, Jae-Sung Kwon, Eunjung Lee, Seunghan Oh, Eun Ha Choi, Kyoung Nam Kim |
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| Rok vydání: | 2016 |
| Předmět: |
Nanotube
Materials science Simulated body fluid Biomedical Engineering Pharmaceutical Science Medicine (miscellaneous) Bioengineering 02 engineering and technology Sputter deposition Plasma electrolytic oxidation 010402 general chemistry 021001 nanoscience & nanotechnology 01 natural sciences 0104 chemical sciences Ion Contact angle X-ray photoelectron spectroscopy Chemical engineering Sputtering General Materials Science 0210 nano-technology |
| Zdroj: | Journal of Biomedical Nanotechnology. 12:1890-1906 |
| ISSN: | 1550-7033 |
| DOI: | 10.1166/jbn.2016.2310 |
| Popis: | We prepared TiO2 nanotubes (NT) on commercially pure titanium (cp-Ti) substrate by plasma electrolyte oxidation and adapted magnetron sputtering for incorporation of Ag-nanoparticles (Ag-NPs) onto the nanotubes (Ag-NPs/TiO2 nanotube). Power input to the Ag target per unit time was varied (5, 10, 15 W/cm2) to fabricate different shapes of Agnanoparticles onto the nanotubes while net energy input was fixed by maintaining a constant total sputter time (30, 15, 10 s, respectively). For investigation of experimental samples' characteristics, FE-SEM, TEM, EDS, XRD, XPS, SPM analysis and contact angles measurement was carried out. Through these characterization, plasma engineered Ag-NPs was successfully formed on/in the entire nanotube structure. In terms of antibacterial ability, plasma engineered Ag-NPs/TiO2 nanotubes samples significantly reduced S. aureus colony numbers compared with control. Also, simulated body fluid immersion tests with hydroxyapatite showed ion precipitation onto the surface of all experimental groups, confirmed by XRD and EDS analysis. However, plasma engineered Ag-NPs/TiO2 nanotubes groups were not cytotoxic. Furthermore, MC3T3-E1 cells were cultured on Ag-NPs/TiO2 nanotubes groups to evaluate the effect of nanostructured surface on cell functionality such as a cell proliferation and ALP activity. Ag-NPs/TiO2 nanotubes have both biocompatible and antibacterial characteristics. |
| Databáze: | OpenAIRE |
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