Radioimmunoassay of factor V in human plasma and platelets
| Autor: | Kenneth G. Mann, Lisa L. Eide, E J Bowie, Paula B. Tracy |
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| Rok vydání: | 1982 |
| Předmět: | |
| Zdroj: | Blood. 60:59-63 |
| ISSN: | 1528-0020 0006-4971 |
| Popis: | Homogeneous, single-chain human factor V was used to develop a double antibody competition radioimmunoassay to measure factor V concentrations in plasma and platelets. Standard curves were constructed that allow for the detection of as little as 20 ng factor V/ml of plasma. Normal factor V concentrations range from 4 to 14 micrograms/ml of plasma with an average value of 7.0 +/- 2.0 micrograms/ml (n = 64). No correlation was observed between antigen levels and age or sex. The radioimmunoassay data are consistent with factor V clotting assays, providing freshly drawn plasma is used in the bioassay. Radioimmunoassay of washed platelets indicate that 0.63–1.93 microgram of factor V is present per 2.5 X 10(8) platelets (4612–14128 molecules of the factor V platelet). When normalized to individual hematocrits and platelet count, the data indicated that platelets contribute approximately 18%-25% of the factor V found in whole blood. In addition, two individuals with functionally deficient factor V were examined and found to be deficient in both antigen and activity. |
| Databáze: | OpenAIRE |
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