Deagglomeration and characterization of detonation nanodiamonds for biomedical applications
| Autor: | Andrei Sarabia-Sainz, Raúl Riera, Martín Pedroza-Montero, Mónica Acosta-Elías, Karla Santacruz-Gómez, Noralvis Fleitas-Salazar, Seidy Pedroso-Santana |
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| Rok vydání: | 2017 |
| Předmět: |
Materials science
Health Toxicology and Mutagenesis Biomedical Engineering Analytical chemistry Detonation chemistry.chemical_element 02 engineering and technology 010402 general chemistry 01 natural sciences General Biochemistry Genetics and Molecular Biology symbols.namesake X-ray photoelectron spectroscopy Dynamic light scattering Artificial Intelligence General Pharmacology Toxicology and Pharmaceutics Fourier transform infrared spectroscopy Nanodiamond Zirconium General Immunology and Microbiology General Neuroscience General Medicine 021001 nanoscience & nanotechnology 0104 chemical sciences chemistry Transmission electron microscopy symbols 0210 nano-technology General Agricultural and Biological Sciences Raman spectroscopy |
| Zdroj: | Journal of Applied Biomedicine. 15:15-21 |
| ISSN: | 1214-0287 1214-021X |
| Popis: | Detonation nanodiamonds (DNDs) are usually small particles of 4–5 nm, but in aqueous suspension, DNDs form agglomerates in sizes larger than 1 μm. We propose the use of Bead Assisted Sonic Disintegration and a carboxylation procedure, to reduce DNDs aggregates sizes to approximately 100 nm. High cost zirconium beads have been substituted by silica beads synthetized in our laboratory and less-time consuming conditions were standardized. Techniques as Dynamic Light Scattering (DLS), Fourier Transform InfraRed Spectroscopy (FTIR), Transmission Electron Microscopy (TEM) and X-ray Photoelectron Spectroscopy (XPS), have been used to characterize the resulting diamond nanoparticles. While the incubation of Red Blood Cells with partially disaggregated DNDs was used to study whether these nanodiamonds impact in a living system. Our results show the absence of a negative effect in cell viability as well as no differences between Raman spectra of hemoglobin (Hb), from control and cell + DNDs conditions. |
| Databáze: | OpenAIRE |
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