Mechanistic studies of proteases and lipases for the detergent industry
| Autor: | Erik Gormsen, Henrik Malmos, Dorrit Anita Aaslyng |
|---|---|
| Rok vydání: | 2007 |
| Předmět: |
chemistry.chemical_classification
Proteases Protease Chromatography biology Renewable Energy Sustainability and the Environment General Chemical Engineering medicine.medical_treatment Organic Chemistry Pollution Inorganic Chemistry Fuel Technology Enzyme chemistry Biochemistry Ionic strength medicine biology.protein Lipase Waste Management and Disposal Biotechnology |
| Zdroj: | Journal of Chemical Technology & Biotechnology. 50:321-330 |
| ISSN: | 1097-4660 0268-2575 |
| DOI: | 10.1002/jctb.280500304 |
| Popis: | The wash performance of three specific proteases (asp, lys/arg and glu specific, respectively) is demonstrated to be comparable to that of commercial unspecific detergent proteases (Alcalase® and Savinase®). A hypothesis for the high performance of these specific proteases is proposed. The influence of pH and ionic strength on protease performance is discussed. The removal of oily stains by lipases in detergent solution is improved only slightly under acidic conditions, whereas pH values >> pKa of the fatty acids formed results in strongly enhanced removal. Wash trials with LipolaseTM show very little effect of the lipase after the first wash, but high effect after the second and subsequent washes. This is explained by lipolytic activity under drying. Maximum activity is found when the water content of the cotton swatches is between 10 and 40% (w/w). |
| Databáze: | OpenAIRE |
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