Efficient Biochemical Cascade for Accessing Green Leaf Alcohols
| Autor: | Bojan Bosijokovic, Fredi Brühlmann |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
chemistry.chemical_classification biology 010405 organic chemistry Microorganism Organic Chemistry food and beverages Fatty acid Lyase 01 natural sciences Yeast 0104 chemical sciences Catalysis 03 medical and health sciences Lipoxygenase 030104 developmental biology Enzyme chemistry Cascade reaction biology.protein Organic chemistry Physical and Theoretical Chemistry |
| Zdroj: | Organic Process Research & Development. 20:1974-1978 |
| ISSN: | 1520-586X 1083-6160 |
| Popis: | The biotechnological production of six-carbon membered green leaf aldehydes and alcohols appreciated for their fruity, green-grassy odors in fragrances and flavors exploits the 13-lipoxygenase pathway found in higher plants. Homogenized seeds, fruits, or wild-type microorganisms have traditionally served as catalysts used in a one-pot reaction. However, many of these catalysts can be advantageously replaced by enzymes produced in engineered microorganisms for improved process performance. Substituting guava fruits by an engineered 13-hydroperoxide lyase produced in E. coli was recently shown to provide increased efficiency for cleaving fatty acid 13-hydroperoxides into green leaf aldehydes. Replacing baker’s yeast by a cosubstrate-dependent recombinant ketoreductase was found to offer superior productivity and chemo-selectivity in the reduction of hexenals to the corresponding alcohols. Finally, combining both cellular catalysts at reasonable loading in a simple one-pot cascade reaction offered the green ... |
| Databáze: | OpenAIRE |
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