Evaluation of vascular endothelial growth factor gene and protein expression in canine metastatic mammary carcinomas
| Autor: | Ivan Jose Vechetti-Junior, Erika M. Terra, Silvia Regina Rogatto, Renée Laufer-Amorim, Rosana da Cruz Lino Salvador, Mirela Tinucci-Costa, Talita M. M. Raposo-Ferreira, Juarez Henrique Ferreira |
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| Rok vydání: | 2016 |
| Předmět: |
Pathology
medicine.medical_specialty Histology 040301 veterinary sciences Mammary gland law.invention Metastasis 0403 veterinary science 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine law medicine Gene and protein expression Instrumentation Gene Polymerase chain reaction business.industry 04 agricultural and veterinary sciences medicine.disease Endothelial stem cell Vascular endothelial growth factor Medical Laboratory Technology medicine.anatomical_structure chemistry 030220 oncology & carcinogenesis Cancer research Immunohistochemistry Anatomy business |
| Zdroj: | Microscopy Research and Technique. 79:1097-1104 |
| ISSN: | 1059-910X |
| DOI: | 10.1002/jemt.22763 |
| Popis: | Vascular endothelial growth factor (VEGF) is a potent angiogenic factor that could be associated with the induction of endothelial cell proliferation and metastasis. In this study, we evaluated VEGF gene and protein expression in canine mammary tumors (CMT), including metastatic carcinomas, to determine if there is an influence of this marker in the malignant processes and aggressiveness of CMT. We also compared VEGF protein levels with clinicopathological features. The VEGF gene and protein expression levels were evaluated by reverse transcription-quantitative polymerase chain reaction and immunohistochemistry, respectively, in normal mammary gland samples, benign mammary tumors, nonmetastatic mammary carcinomas, and metastatic mammary carcinomas. High VEGF gene and protein levels were associated with malignant tumors compared with normal mammary glands (p = 0.0089 and p |
| Databáze: | OpenAIRE |
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