Mechanisms of radiation-induced craniofacial bone growth inhibition and possibilities of cytoprotection
| Autor: | La Scala, Giorgio |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2012 |
| Předmět: |
Craniofacial bone growth
Male Bone Development/*drug effects/*radiation effects Histology Cephalometry Histopathology Osteoblasts/drug effects/radiation effects Facial Bone/radiation effects Zygoma/drug effects/radiation effects Random Allocation Amifostine Orbit/growth & development/radiation effects/radiography Radiation Injuries Experimental/*physiopathology Skull/growth & development/radiation effects Growth/radiation effects Radiation/adverse effects Animals Radiation-Protective Agents/*pharmacology Radiation Injuries Dose Fractionation Cells Cultured Hemodynamics/*radiation effects Periosteum/cytology/drug effects/radiation effects Radiation protection Radiation-protective agents Zygoma Bone Development Radiation Zygoma/growth & development/radiation effects/radiography Head and Neck Neoplasms/radiotherapy Rabbits/radiation effects Skull/growth and development Facial Bone/growth and development Orbit/drug effects/radiation effects Cytoprotection Amifostine/*pharmacology Radiation-Protective Agents/pharmacology/*therapeutic use Models Animal Amifostine/pharmacology/*therapeutic use Radiation Protection/drug effects Facial Bones/drug effects/*growth & development/*radiation effects Rabbits Tomography X-Ray Computed Orbit Mandible/radiography |
| DOI: | 10.13097/archive-ouverte/unige:21690 |
| Popis: | Malignancies are frequently located in the head and neck region in children; their management usually requires radiotherapy, which has severe consequences on the growth of the craniofacial skeleton. The purpose of this work is to investigate the effects of radiation in the growing craniofacial skeleton in a rabbit model in vivo and in vitro and the possibilities of growth preservation using the cytoprotective drug Amifostine. The effects of radiation were studied in a single-dose radiation model, then in a more clinically-relevant fractionated-dose radiation model. Both radiation administration modes produced significant bone growth impairment. Administration of Amifostine prior to radiation significantly preserved craniofacial growth; this did not appeared to be related to vascularity as we did not demonstrated histomorphometric vessel changes or long-lasting changes in blood flow. Amifostine- treated animals had increased osteoblast counts, and improved bone turn-over compared to unprotected animals. In vitro, Amifostine enhanced the number of surviving cells after irradiation. This work suggests that radioprotection offers a viable pathway for the prevention of radiation-induced craniofacial bone growth inhibition and ensuing deformities. |
| Databáze: | OpenAIRE |
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