Near-infrared fluorescent probes for detection of exogenous and endogenous hypochlorite in living cells
| Autor: | Yu Chen, Jun Feng Zhang, Ping Xie, Yumin Wang, Lina Huang, Ying Zhou, Xiang Hua Wu, Yu Qiang Zhao |
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| Rok vydání: | 2020 |
| Předmět: |
Detection limit
biology Hypochlorous acid Process Chemistry and Technology General Chemical Engineering Hypochlorite Endogeny 02 engineering and technology 010402 general chemistry 021001 nanoscience & nanotechnology biology.organism_classification 01 natural sciences Fluorescence 0104 chemical sciences HeLa chemistry.chemical_compound Membrane chemistry In vivo Biophysics 0210 nano-technology |
| Zdroj: | Dyes and Pigments. 177:108308 |
| ISSN: | 0143-7208 |
| Popis: | Hypochlorous acid (HClO)/hypochlorite (ClO−) play crucial roles in many physiological and pathological processes. Herein, two near-infrared fluorescent probe FDOCl-1 and FDOCl-2 were designed and synthesized for selective and sensitive detecting ClO−. The probe showed fast response (FDOCl-1: less than 50 s, FDOCl-2: less than 30 s), remarkable colorimetric change, excellent selectivity and sensitivity, low limit of detection (FDOCl-1: 53.5 nM, FDOCl-2: 31.7 nM) and good pH stability. Moreover, the probe FDOCl-2 could penetrate cell membranes, and was used to detect exogenous and endogenous ClO− in living HeLa cells and Caenorhabditis elegans. Thus, we suggest that our newly generated probe FDOCl-2 might have potential application in the rapid and sensitive detection of ClO− in vivo. |
| Databáze: | OpenAIRE |
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