Potential Antimicrobial Effects of Gatifloxacin on Periodontopathic Bacteria in Vitro
| Autor: | Tadashi Miura, Masataka Kasahara, Koichi Kida, Nahoko Miyake, Koji Tanabe, Yasuhiko Shizawa, Masao Yoshinari, Eri Tsukagoshi |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
Peri-implantitis
biology Chemistry Aggregatibacter actinomycetemcomitans Prevotella intermedia 030206 dentistry bacterial infections and mycoses biology.organism_classification Antimicrobial Gatifloxacin In vitro Microbiology Dermal fibroblast 03 medical and health sciences 0302 clinical medicine 030221 ophthalmology & optometry medicine heterocyclic compounds Bacteria medicine.drug |
| Zdroj: | Journal of Biomedical Science and Engineering. :354-359 |
| ISSN: | 1937-688X 1937-6871 |
| Popis: | The aim of this study was to identify the potential antibacterial effects of gatifloxacin on periodontal pathogens including Aggregatibacter actinomycetemcomitans, Porphyromonas gingi-valis, and Prevotella intermedia. The minimum inhibitory concentrations (MIC) of gatifloxacin and its bactericidal effects were investigated. Gatifloxacin inhibited the growth of all three kinds of periodontopathic bacteria tested in broth. The MIC value of 2.5 nM was found to be the most effective in inhibiting A. actinomycetemcomitans. An adenosine triphosphate biolumi-nescence assay revealed that gatifloxacin exhibited bactericidal effects on the tested bacteria in a time-dependent manner. The safety of gatifloxacin in mammalian cells was evaluated by assessing the viability of normal human dermal fibroblast (NHDF) cells treated with gatifloxacin. Almost all NHDF cells survived after 2-d culture, while 81% of the cells survived after 4-d culture when treated with 1.0 × 103 nM gatifloxacin. These results indicate that gatifloxacin is a possible drug for local administration to prevent periodontal infection. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|