| Autor: |
Kristyn S. Masters, Chloe M. McCoy, Dylan Q. Nicholas |
| Rok vydání: |
2012 |
| Předmět: |
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| Zdroj: |
5th Biennial Conference on Heart Valve Biology and Tissue Engineering. |
| Popis: |
Although the pathogenesis of calcific aortic valve disease (CAVD) is not well understood, males have been identified as having a two-fold increased risk for the disease when compared to females. In this study, we examined gene expression profiles in healthy pigs and measured markers of disease in vitro to determine whether the differences in clinical risk between males and females translate into measurable intrinsic differences on the cellular scale. In addition, we also investigated potential sex-related differences in cellular response to TGF-β1, an inflammatory stimulus known to be elevated in calcified human aortic valve explants. mRNA was isolated from three male and three female porcine aortic valves (denuded of endothelial cells) and hybridized to Affymetrix® GeneChip Porcine Genome microarrays. Mean expression values of each probe set in the male samples were compared with those in the female samples and biological processes were analyzed from the dataset for overrepresentation using Gene Ontology term enrichment analysis. From the microarrays there were 183 genes identified as being significantly (fold change>2; P |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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