Liquid chromatography with luminol-based electrochemiluminescence detection

Autor:	H. Lingeman, D.A. Kamminga, A Brummelhuis, O. M. Steijger
Rok vydání:	1998
Předmět:	Detection limit Analyte Working electrode Chromatography Organic Chemistry General Medicine Biochemistry High-performance liquid chromatography Analytical Chemistry Luminol chemistry.chemical_compound chemistry Reagent Electrochemiluminescence Derivatization
Zdroj:	Journal of Chromatography A. 799:57-66
ISSN:	0021-9673
DOI:	10.1016/s0021-9673(97)01083-2
Popis:	The liquid chromatographic determination of histamine was achieved by precolumn derivatization with N-(4-aminobutyl)-N-ethylisoluminol isothiocyanate and electrochemiluminescence detection. Detection was carried out using postcolumn on-line electrochemical reagent generation and oxidation of the derivatized analyte at a gold gauze working electrode built in the flow cell of the detector. With this on-line method a detection limit of 1.5 pmol (S/N53) of histamine was obtained. The method was linear in the range 0.5–10 nmol and the repeatability of the method was satisfactory (R.S.D.513.7% and 3.7% at the 0.5 and 5 nmol level, respectively).
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::b23175c9e0401047f6e86017450a4588 https://doi.org/10.1016/s0021-9673(97)01083-2 Zobrazit plný text záznamu Full Text from ScienceDirect