AMPA receptor imaging by infrared scanning near‐field optical microscopy

Autor:	Johanna Generosi, Harald Hirling, Giorgio Margaritondo, Kai Johnsson, Norman Tolk, A. Cricenti, David W. Piston, Stefan Catsicas, Michel Kropf
Rok vydání:	2008
Předmět:	Fluorophore Chemistry Infrared business.industry Infrared spectroscopy Near and far field AMPA receptor Condensed Matter Physics Photobleaching law.invention Wavelength chemistry.chemical_compound Optics nervous system Optical microscope law Biophysics business
Zdroj:	physica status solidi c. 5:2641-2644
ISSN:	1610-1642 1862-6351
Popis:	Infrared scanning near-field optical microscopy is used to detect AMPA-type glutamate receptor clusters in hippocampal neuron cells. AMPA's GluR2-subunits were marked with the fluorophore Alexa488 and high-resolution micrographs were taken at 6.25 µm wavelength, corresponding to the maker's strong infrared absorption. The use of infrared light avoids photobleaching and makes this approach suitable for long-term observations; this technique can be used in general to localize different IR-absorbing molecules over the complex background of other cell components.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::b228f2c82a5ef5418d9ce916a72ba6df https://doi.org/10.1002/pssc.200779116 Zobrazit plný text záznamu Plný text