cDNA macroarray analysis of gene expression changes in rat brain after a single administration of a 2-aminoadamantane derivative

Autor:	Yu. V. Vakhitova, S. B. Seredenin, R. S. Yamidanov, V. A. Vakhitov
Rok vydání:	2005
Předmět:	chemistry.chemical_classification biology Biophysics Molecular Pharmacology Molecular biology Enzyme chemistry Structural Biology Complementary DNA Gene expression biology.protein Cytoskeleton Gene Actin Glyceraldehyde 3-phosphate dehydrogenase
Zdroj:	Molecular Biology. 39:244-252
ISSN:	1608-3245 0026-8933
Popis:	The Atlas™ Rat cDNA Expression Array (BD Biosciences, United States) has been used to analyze changes in the expression of 588 genes in rat brain cells in response to a single administration of Ladasten, a 2-aminoadamantane derivative that has psychostimulating and anxiolytic effects. The analysis of hybridization on macroarrays, confirmed by the results of real-time quantitative RT-PCR, has demonstrated that Ladasten alters the expression of 12 genes in the rat brain. The GAT3 and CARBH genes are presumed to be pharmacologically important targets of Ladasten. The changes in their activity explain the mechanisms of the anxiolytic and mood-stabilizing effects of the drug. Ladasten has been shown to induce the genes whose products are involved in various signal pathways (APC, Rb, PKCIP, and PMCA), as well as the genes of cytoskeletal proteins (Tubα1 and actin), synaptic proteins (SynIA&IB and PLP), and enzymes (Gapdh and NSE). The proteins encoded by these genes are presumably involved in compensatory and/or neuroplastic adaptation to the effects of Ladasten.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::b201613843a1d6b1adb3eab964f36d92 https://doi.org/10.1007/s11008-005-0035-7 Zobrazit plný text záznamu Full text from SpringerLink