Effect of Electrophoresis on the Efficiency of Graphite-Nano-TiO2 Modified Silica Sol–Gel Electrode

Autor:	Sima Gholizadeh, Mir Reza Majidi, S. M. Golabi, Anthony Turner, Mohammad Saeid Hejazi, Ezat Hamidi-Asl
Rok vydání:	2015
Předmět:	Detection limit Materials science Chromatography Tio2 nanoparticles Biomedical Engineering Bioengineering General Chemistry Nano tio2 Condensed Matter Physics chemistry.chemical_compound Electrophoresis chemistry Lactate dehydrogenase Electrode General Materials Science Graphite Sol-gel
Zdroj:	Journal of Nanoscience and Nanotechnology. 15:3405-3410
ISSN:	1533-4899 1533-4880
DOI:	10.1166/jnn.2015.10215
Popis:	Electrophoresis treatment was used to improve the function of a nano-TiO2 modified sol-gel electrode. Electrodes were prepared using TiO2 nanoparticles and fine graphite powder and then treated by electrophoresis. The developed electrode was employed for the detection of lactate dehydrogenase (LDH) by following the decrease in the immobilised lactate peak current due to its LDH-mediated enzymatic oxidation. Detection was realised using square wave voltammetry (SWV). Experiments showed that the positive and negative heads of the electrophoresis-treated electrode displayed different activities, with the positive head response being remarkably improved. Parameters affecting the electrode response, such as applied potential value, electrophoresis time and percentage of TiO2, were investigated and optimised. The improved performance was dependent on TiO2 concentration as well as electrophoresis voltage and time. The prepared sensor, under optimised conditions, displayed a detection limit of 0.0073 U/μl for LDH.
Databáze:	OpenAIRE
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