| Autor: |
Franciane Cristina de Figueiredo, Tania Sila Campioni, Pedro de Oliva Neto, Ana Flávia Azevedo Carvalho, Douglas Fernandes Silva |
| Rok vydání: |
2020 |
| Předmět: |
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| Zdroj: |
International Journal of Environment, Agriculture and Biotechnology. 5:217-230 |
| ISSN: |
2456-1878 |
| Popis: |
Sugarcane straw (SS) was used in an economic biosystem to evaluate the production of xylanases and cellulases in submerged fermentation (SmF) by axenic and mixed mode from Trichoderma and Aspergillus species. T. reesei QM9414 axenic culture reached the highest xylanase production (90.2 U/mL) and 0.5 FPU/mL of cellulase activity. The evaluation of agro-industrial residues on fibrolytic enzymes production was performed by a D-optimal design, and revealed the best supplementation of 100% SS, while wheat bran and citric pulp showed lower inductive effects on enzymes production. Also, the scale-up in a stirred tank showed the same yield production profile (xylanase ~ 90 U/mL and celullase 0.6 FPU/mL). Xylanase was characterized by an optimum pH of 5-6 and temperature at 50 oC, and thermal stability was below 50 oC. The ion Mn2+ (5 and 10 mM) had a stimulatory effect on xylanase activity. The biobleaching application showed that 30 U/g of xylanases during 15 min decreased Kappa number in 9.37. These results indicate SS as an alternative substrate for fungi fibrolytic enzymes production and the xylanase with low cellulase extract as a potential biobleaching application. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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