Adenylate cyclase activity in sheep platelets following external whole-body gamma-irradiation with different doses
| Autor: | T.S. Shevchenko, V.O. Kobyalko |
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| Rok vydání: | 2020 |
| Předmět: | |
| Zdroj: | "Radiation and Risk" Bulletin of the National Radiation and Epidemiological Registry. 29:136-145 |
| ISSN: | 2412-950X 0131-3878 |
| DOI: | 10.21870/0131-3878-2020-29-4-136-145 |
| Popis: | The paper presents results of study of adenylate cyclase activity in the platelets after external whole-body gamma-irradiation of 32 tsigai rams (Ovis aries) with 2, 4 and 6 Gy, at the dose rate of 1 Gy/hour. The irradiation caused the development of acute radiation damage of a different degree of severity. To detect changes in the basal and prostoglandin E1 stimulated (PGE1-stimulated) adenylate cyclase activity thin-layer chromatography (TLC) was carried out during the first 30 days of the damage development. After external -irradiation directly proportional effect of dose on changes in the basal adenylate cyclase activity in the platelets was registered on the 1 and the 7 days of the damage development, the changes in the PGE1-stimulated activity was registered on the 7 day. The study results allow making the suggestion that detected changes in the adenyl-ate cyclase activity in the platelets of irradiated rams may be resulted from the modification of cy-toplasmic cell membranes and impaired functioning of membrane-bound cAMP-sensitive signal-ing pathway. It may be also caused by new cells with qualitatively new properties released from the bone marrow megakaryocytes pool into the bloodstream. |
| Databáze: | OpenAIRE |
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